Purpose This study evaluates the effect of uptake times (2.5 hours vs. 3.5 hours post-injection) on the diagnostic quality of wholebody bone scans using ^99mTc -DPD, focusing on contrast between bone and soft tissue. The aim is to determine whether shorter uptake times can ensure sufficient diagnostic quality while enhancing patient convenience and clinical workflow efficiency, particularly during periods of radiopharmaceutical shortages. Materials and Methods A total of 60 patients, divided into a normal group (n=30) and a lesion group (n=30), were included in this study. All scans were performed using Siemens gamma cameras equipped with LEHR collimators. Scans were conducted at 2.5- and 3.5-hours post-injection. Regions of interest (ROIs) were defined for femoral bone and adjacent soft tissue to calculate uptake ratios. Statistical analysis was performed using paired t-test to compare uptake ratios. Imaging conditions and radiopharmaceutical doses were carefully standardized across all patients to minimize variability. Results In the normal group, the mean uptake ratio increased from 70.1% ± 4.7% at 2.5 hours to 72.6% ± 4.3% at 3.5 hours, showing a statistically significant difference (p < 0.05). Similarly, in the lesion group, uptake ratios improved from 90.7% ± 4.3% to 92.7% ± 3.9%, also demonstrating statistical significance (p < 0.05). While the 3.5-hour uptake time produced slightly better lesion contrast and improved diagnostic utility, the 2.5-hour scans provided comparable results in visual quality and diagnostic accuracy, making them a viable option in resource-constrained environments. Conclusion The findings suggest that while a 3.5-hour uptake time yields optimal diagnostic quality and superior lesion contrast, the 2.5-hour time point remains a practical alternative, especially in high-demand clinical settings or during radiopharmaceutical supply constraints. This study supports the development of flexible, optimized bone scan protocols that maintain high diagnostic standards without compromising operational efficiency.