Introduction: Constipation is one of the common modern diseases today. Distinguishing between primary and secondary (organic) constipation is essential for appropriate treatment. In 1999, Dylan Bartholomeuse et al. conducted a comparative study of Ga-67 and In-111, finding that the results of both were very similar. This is good news for Nuclear Medicine in Taiwan, as Ga-67 is more accessible and cheaper than In-111. Therefore, the evaluation metrics of our department also refer to the conclusions of this study. Material and Methods: Our clinical procedure involves patients ingesting 300 c.c. of water or orange juice mixed with 1 mCi Ga67, followed by imaging at 6, 24, 48, and 72 hours. Reviewing patients from 2020 to 2025, there were a total of 40 patients who underwent colon transit study in our department, ranging in age from 20 to 73 years (with an average age of 44.3 years). Results: Among them, 15 were normal patients with an average half-clearance time of 21.72 hours, and 24 were abnormal patients with an average half-clearance time of 88.25 hours. Additionally, one patient was unable to complete the scan for personal reasons. Imaging from normal patients showed the activity of the isotope in the colon: the ascending colon (A) became visible at 6 hours and had almost no radioactive residue after 72 hours; the transverse colon (T) and descending colon (D) were visible around 24 to 48 hours; and the sigmoid colon (S) and rectum (R) were visible from 24 to 72 hours. Conclusions: Nuclear medicine offers significant advantages in the diagnosis of constipation, providing clinicians with critical functional information that enables more accurate identification of the underlying causes and the development of appropriate treatment strategies.